Published: 25 July, 2017 | Volume 1 - Issue 1 | Pages: 019-036
Figure 3:
The CRISPR / Cas9 system. The DNA target sequence (orange) is illustrated with the two strands separated. The sense strand of target sequence id followed at its 3’ end by the protospacer adjacent motif (PAM), which is NGG (in blue) for the SpCas9 nuclease. The cleavage site (double arrow in red) is located at 3 nucleotides (CGG in mauve) from the PAM towards the 5 ‘end. The variable portion of sgRNA (20 nucleotides in green) recognizes the target sequence by the Watson-Crick base pairing. The nuclease Cas9 (in light gray) is recruited at the PAM. The invariable part of sgRNA (nucleotides in black) stabilizes the whole structure. Image modified from [107].
Read Full Article HTML DOI: 10.29328/journal.jgmgt.1001003 Cite this Article Read Full Article PDF
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